KG-1

86111306, human bone marrow, Myeloblast-like

• Sinónimos: KG1 Cells
• Código UNSPSC: 41106514

Propiedades

• product name: KG-1, 86111306
• origen biológico: human bone marrow
• modo de crecimiento: Suspension
• cariotipo: 2n = 46, diploid
• morfología: Myeloblast-like
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian bone marrow myelogenous leukaemia

Descripción línea celular

Derived from the bone marrow aspirate of a 59 year old male Caucasian with erythroleukemia that became acute myelogenous leukaemia. The cells form colonies in soft-agar in the presence of colony stimulating factor (CSF). KG-1 cells resemble acute myelogenous leukaemia showing considerable pleomorphism with a predominance of myeloblasts and promyelocytes. A small percentage of the cells are mature granulocytes with the occasional presence of macrophages and eosinophils. They do not express surface Ig or EBV antigens and lack specific markers for lymphocytic cells.

Aplicación

KG-1 has been used to extract total RNA and use them as templates to determine the 5′ end of promyelocytic leukemia zinc finger (PLZF) transcripts.

Perfil del ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19

Medio de cultivo

IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Otras notas

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