BC3H1

86093001, mouse brain, Fibroblast

• Código UNSPSC: 41106514

Propiedades

• product name: BC3H1, 86093001
• origen biológico: mouse brain
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Fibroblast
• productos: Acetylcholine
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse brain tumour

Descripción línea celular

Established in 1974 from a mouse brain neoplasm tumour induced by nitrosoethylurea, possess many properties characteristic of smooth muscle. Since then it has been suggested that BC3H1 is a skeletal line of ectodermal origin that is defective for commitment to terminal differentiation. The two distinct morphologies typical of this cell line are large spheroid cells and smooth-muscle-like cells. The fusiform is the more common.

Aplicación

Acetylcholine receptor studies.

Medio de cultivo

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Spilt confluent cultures 1:3 to 1:10 i.e. seeding at 1-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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