EL4 Cell Line murine

Lymphoblast from blood, 85023105

• Código UNSPSC: 41106514

Propiedades

• origen biológico: mouse blood
• modo de crecimiento: Suspension
• cariotipo: 2n = 39
• morfología: Lymphoblast
• productos: A surface antigen induced by leukaemia type G virus; H-2b and Thy-1.2 antigens; Interleukin 4 (IL-4), H-2b, Thy1.2
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse ascites lymphoma lymphoblast

Descripción línea celular

Established from a lymphoma induced in a C57BL/6N mouse by 9,10-dimethyl-1,2-benzanthracene. The cells have been reported to produce low levels of type G (Gross) virus antigen histone-2b (H-2b), interleukin-4 (IL-4) and Thy-1.2 antigen. The cell line is resistant to cortisol and dexamethasone and is sensitive to polyhydroxyalkanoates (PHAs).

Aplicación

EL4 Cell Line murine has been used to induce tumor in mice and to assess the extravasation of tumor necrosis factor (TNF)-induced Evans blue dye and E1- and E3-deleted Ad vectors carrying firefly luciferase reporter gene under cytomegalovirus promoter (AdLuc). It has also been used as a constituent of bone marrow-derived macrophage (BMDM)-polarizing media to polarize BMDM and assess its tumoricidal activity.

Tumourigenicity studies

Medio de cultivo

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cell growth after resuscitation is slow. Subculture once exponential growth is established.

Otras notas

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