LIM2099 cell line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 12062002, human liver (metastasis), epithelial

• Sinónimos: LIM 2099, LIM-2099
• Código UNSPSC: 41106514

Propiedades

• origen biológico: human liver (metastasis)
• envase: tube of 5 μg 12062002-DNA-5UG; pkg of vial of cells 12062002-1VL
• modo de crecimiento: adherent
• morfología: epithelial
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Descripción línea celular

Human liver metastasis, moderately differentiated sclerosing adenocarcinoma. Cell Bank Australia reference number: CBA0164.

Medio de cultivo

RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10μM

Rutina de subcultivo

Optimal split ratio 1:4-1:6 (seeding density 0.8 x 10⁴ cells/cm²). Cells incubated at 37°C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5-10 min.

Otras notas

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