MKL-1

09111801, human skin. Loose floating aggregates with irregular outline and no central necrosis.

• Sinónimos: MKL1 Cells
• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: MKL-1, from human skin, 09111801
• origen biológico: human skin
• modo de crecimiento: Suspension
• cariotipo: Hyperdiploid
• morfología: Loosely packed floating aggregates with irregular outline and no central necrosis
• productos: MKL-1 is positive for CM2B4/MCV T AE1/AE2, CK20, EMA, CAM5.2, NSE and TTF-1. MKL-1 is negative for CK7, chromagen, synaptophysin, neurofilament, LCA, vimentin and CD99.
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: metastasis
• Condiciones de envío: dry ice

Descripción

Origen línea celular

Human neuroendocrine skin carcinoma

Descripción línea celular

MKL-1 was established from a nodal metastasis in a 26-year-old Caucasian male patient. MKL-1 cells contain a Merkel cell polyomavirus integrated into the genome. MKL-1 cells cause tumour formation at 4-6 weeks at subcutaneous sites in athymic male nude mice (BALB/c nu/nu).

Perfil del ADN

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 10,12
D5S818: 11,12
D7S820: 8,11
THO1: 9,9.3
TPOX: 8
vWA: 16,18

Medio de cultivo

RPMI 1640 + 2 mM Glutamine + 10% FBS

Rutina de subcultivo

Seed at 3 x 10⁵ cells/ml in 5% CO₂; 37 °C Saturation density is approximately 10⁶ cells/ml. Population doubling time is around 120 hours.

Otras notas

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