h1RPE7

9061602, human eye, Polygonal

• Código UNSPSC: 41106514

Propiedades

• product name: h1RPE7, 09061602, from human eye
• origen biológico: human eye
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Polygonal
• productos: Cytokeratins 5 and 8, junctional protein zonula occludens (ZO-1)
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice

Descripción

Origen línea celular

An immortalized retinal pigment epithelial cell line

Descripción línea celular

The h1RPE7 cell line was derived from primary human retinal pigment epithelial cells obtained from a 50-year-old female Caucasian donor. The primary culture was transfected with a construct encoding SV40 large T antigen that was deleted for the small t antigen along with puromycin resistance gene.

Perfil del ADN

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,13
D13S317: 12
D16S539: 12
D5S818: 9,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,19

Medio de cultivo

Hams F10 + 20% Heat-inactivated FCS + 2 mM Glutamine + selection antibiotic 1μg/ml puromycin.

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 24hrs.

Otras notas

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Cláusula de descargo de responsabilidad

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.