A9(Hamprecht)

8062526, mouse connective tissue, Fibroblast

• Código UNSPSC: 41106514

Propiedades

• product name: A9(Hamprecht), 08062526
• origen biológico: mouse connective tissue
• modo de crecimiento: Adherent
• cariotipo: Modal No of chromosomes is 54
• morfología: Fibroblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse Fibroblast (L cells)

Descripción línea celular

A 6-thioguanine resistant mutant of mouse L cell fibroblasts (Mouse strain C3H/An). A9 lacks guanylic acid-inosinic acid pyrophosphorylase and was isolated by virtue of resistance to 6-thioguanine (3 μg/ml). ECACC has two catalogue entries for the A9 cell line which differ in that they were deposited by different groups: A9 (Sigma Catalogue number 85011426) and A9(Hamprecht) (Sigma Catalogue number 08062526). Several other cell lines in the ECACC catalogue have been derived from A9(Hamprecht).

Aplicación

Used in the generation of cell hybrids.

Medio de cultivo

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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