108CC5

8062521, Mouse x Rat Hybridoma nerve, Neuronal

• Código UNSPSC: 41106514
• origen biológico: Mouse x Rat Hybridoma nerve
• modo de crecimiento: Adherent
• cariotipo: Modal No. varied with passage see reference
• morfología: Neuronal
• productos: Long processes after treatment with dibutyryl cAMP, dense core vesicles, excitable membranes, neurotransmitter enzymes, synthesis of neurohormones and uptake of catecholamines.
• receptores: Receptors for neurohormones: delta opioid, PGE 1 vasoactive intestinal polypeptide, adenosine, somatostatin and acetylcholine.
• enfermedades relevantes: cancer

Propiedades

• Nombre del producto: 108CC5, 08062521
• origen biológico: Mouse x Rat Hybridoma nerve
• modo de crecimiento: Adherent
• cariotipo: Modal No. varied with passage see reference
• morfología: Neuronal
• productos: Long processes after treatment with dibutyryl cAMP, dense core vesicles, excitable membranes, neurotransmitter enzymes, synthesis of neurohormones and uptake of catecholamines.
• receptores: Receptors for neurohormones: delta opioid, PGE 1 vasoactive intestinal polypeptide, adenosine, somatostatin and acetylcholine.
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse neuroblastoma x Rat glioma hybrid

Descripción línea celular

The cell line108CC5 is formed by the fusion of mouse neuroblastoma N18TG2 and rat glioma C6-BU-1using inactivated Sendai virus. This cell line was renamed NG108-5 by Klee and Nirenberg . This is a cholinergic hybrid cell line.

Aplicación

Used as a neuronal Model

Medio de cultivo

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 days

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.

Otras notas

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