HeLa (AC-free)

8011102, human cervix, Spherical

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: HeLa (AC-free), 08011102
• origen biológico: human cervix
• modo de crecimiento: Aggregates in suspension
• cariotipo: 2n = 46, aneuploid
• morfología: Spherical
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human cervical carcinoma, serum-free, animal component (AC) free

Descripción línea celular

HeLa cell line (Sigma catalogue no. 93021013) adapted to grow in animal component free medium (EX-CELL HeLa Serum-Free Medium for HeLa Cells Sigma cat no 14591C).

Aplicación

Virus studies

Medio de cultivo

EX-CELL HeLa Serum-Free Medium (Sigma cat no 14591C)+ 2mM Glutamine. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

Rutina de subcultivo

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran

Otras notas

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