MDCK-SIAT1 Cell Line canine

5071502, canine kidney, Epithelial

• Sinónimos: MDCK-SIAT1 Cells
• Código UNSPSC: 41106514

Propiedades

• product name: MDCK-SIAT1 Cell Line canine, 05071502
• origen biológico: canine kidney
• descripción: Canine Cocker Spaniel Kidney Sialic Acid Over Expression
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial
• productos: Not specified
• receptores: Enhanced expression of 6-linked sialic acids
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Canine Cocker Spaniel Kidney Sialic Acid Over Expression

Descripción línea celular

This cell line has been derived by the stable transfection of MDCK cells with the cDNA of human 2,6-sialtransferase (SIAT1). The cells express two fold?higher amounts of 6-linked sialic acids and two fold-lower amounts of 3-linked sialic acids than parent MDCK cells. MDCK-SIAT1 cells are presented as a suitable system for testing the sensitivity of human influenza virus to neuraminidase inhibitors (NAI) due to their over expression of sialyl-α2,6-galactose moieties.

Medio de cultivo

DMEM + 2mM Glutamine + 1mg/ml G418 + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (85-95 %) 1:10 to 1:20 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO₂.

Otras notas

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