CHSE-214
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 21714, fish embryo, Fibroblast-like
Sinónimos:
CHSE/F
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About This Item
Código UNSPSC:
41106514
Productos recomendados
origen biológico
fish embryo
envase
tube of 5 μg 00021714-DNA-5UG
pkg of vial of cells 00021714-1VL
modo de crecimiento
Adherent
cariotipo
Not specified
morfología
Fibroblast-like
productos
Not specified
receptores
Not specified
técnicas
cell culture | mammalian: suitable
Condiciones de envío
dry ice
temp. de almacenamiento
−196°C
Origen línea celular
Common bluegill (Lepomis macrochirus), normal
Descripción línea celular
The fish line CHSE/F was derived from Common bluegill (Lepomis macrochirus) embryo. It is susceptible to various fish viruses including infectious hematopoietic necrosis virus (IHNV); viral haemorrhagic septicaemia virus (VHSV); spring viraemia of carp virus (SVCV); and infectious pancreatic necrosis virus (IPNV).
ECACC has been advised by the CCLV that this cell line is derived from Lepomis macrochirus (Common bluegill) not Oncorhynchus tshawytscha (Chinook salmon) as originally reported. This has been confirmed by DNA sequencing at ECACC. The CCLV have changed the cell line name to CHSE/F to indicate this. The line is used in viral diagnostics and gives better results than BF-2 (Sigma Catalogue number 00021712), a commonly used cell line of Lepomis macrochirus. The sequence of both lines (BF-2 and CHSE/F possess the same nucleotide exchange but are different in culture behaviour and in virus propagation.
ECACC has been advised by the CCLV that this cell line is derived from Lepomis macrochirus (Common bluegill) not Oncorhynchus tshawytscha (Chinook salmon) as originally reported. This has been confirmed by DNA sequencing at ECACC. The CCLV have changed the cell line name to CHSE/F to indicate this. The line is used in viral diagnostics and gives better results than BF-2 (Sigma Catalogue number 00021712), a commonly used cell line of Lepomis macrochirus. The sequence of both lines (BF-2 and CHSE/F possess the same nucleotide exchange but are different in culture behaviour and in virus propagation.
Aplicación
Fish virus studies
Medio de cultivo
EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 1% Non Essential Amino Acids(NEAA) (M7145) + 120mg/l Sodium Pyruvate(NaP) (S8636) + 10% FBS / FCS (F2442).
Rutina de subcultivo
Split sub-confluent cultures (70-80%) 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA; grow cells in dark (e.g. wrapped in foil) at 20-26ºC with 26ºC optimum temperature; monolayer is stable for at least 10 days with maintenance medium containing 1-2% FBS.
Otras notas
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