C9233
Monoclonal Anti-Chk2 antibody produced in mouse
clone DCS-270, purified from hybridoma cell culture
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About This Item
Productos recomendados
origen biológico
mouse
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
DCS-270, monoclonal
formulario
buffered aqueous solution
mol peso
antigen 61 kDa
reactividad de especies
human
concentración
~1 mg/mL
técnicas
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using using whole extract of cultured 293T (human embryonal kidney) cells
isotipo
IgG2a
Nº de acceso UniProt
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... CHEK2(11200)
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Descripción general
The protein encoded by this gene is a serine/threonine-protein kinase that acts as a cell cycle checkpoint regulator and a putative tumor suppressor. It contains a C-terminal kinase domain, an N-terminal regulatory region that is rich in TQ and SQ pairs, and a forked head-associated domain (FHA), which is activated by rapid phosphorylation in response to replication blocks and DNA damage.
Especificidad
The epitope recognized by the antibody resides within the N-terminal regulatory domain of the Chk2 molecule.
Inmunógeno
recombinant human Chk2.
Aplicación
Monoclonal Anti-Chk2 antibody produced in mouse is suitable for immunocytochemistry, immunohistochemistry (formalin-fixed, paraffin-embedded sections), microarray, immunoprecipitation and for immunoblotting at a concentration of 1-2 μg/mL using whole extract of cultured 293T cells. It was used for Chk2 immunoprecipitation experiments to study the engagement of the ATR-dependent DNA damage response at the human papillomavirus 18 replication centers during the initial amplification
Acciones bioquímicas o fisiológicas
The protein propagates signals from damaged or unreplicated DNA. It is expressed throughout the cell cycle. Upon activation it phosphorylates and inhibits CDC25C, which leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes, blocking cell cycle progression. It also regulates apoptosis through the phosphorylation of p53, a tumor suppressor protein. The protein regulates DNA repair through phosphorylation of BRCA2. Mutations in this gene have been linked to Li-Fraumeni syndrome, sarcomas, breast cancer, and brain tumors.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Yin Liu et al.
Human mutation, 32(9), 1000-1003 (2011-05-28)
The association between the CHEK2 and breast cancer risk in Chinese women is unknown. Here, we screened the full CHEK2 coding sequence in 118 Chinese familial breast cancer cases who are negative for mutations in BRCA1 and BRCA2, one recurrent
Tormi Reinson et al.
Journal of virology, 87(2), 951-964 (2012-11-09)
We have previously demonstrated that the human papillomavirus (HPV) genome replicates effectively in U2OS cells after transfection using electroporation. The transient extrachromosomal replication, stable maintenance, and late amplification of the viral genome could be studied for high- and low-risk mucosal
J Falck et al.
Nature, 410(6830), 842-847 (2001-04-12)
When exposed to ionizing radiation (IR), eukaryotic cells activate checkpoint pathways to delay the progression of the cell cycle. Defects in the IR-induced S-phase checkpoint cause 'radioresistant DNA synthesis', a phenomenon that has been identified in cancer-prone patients suffering from
A L Brown et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(7), 3745-3750 (1999-03-31)
Checkpoints maintain the order and fidelity of the eukaryotic cell cycle, and defects in checkpoints contribute to genetic instability and cancer. Much of our current understanding of checkpoints comes from genetic studies conducted in yeast. In the fission yeast Schizosaccharomyces
Vidar Staalesen et al.
Oncogene, 23(52), 8535-8544 (2004-09-14)
The DNA damage checkpoint kinase, CHK2, promotes growth arrest or apoptosis through phosphorylating targets such as Cdc25A, Cdc25C, BRCA1, and p53. Both germline and somatic loss-of-function CHEK2 mutations occur in human tumours, the former linked to the Li-Fraumeni syndrome, and
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