E4884

Ácido etilenodiaminotetraacético disodium salt dihydrate

ACS reagent, 99.0-101.0%

• Sinónimos: EDTA disodium salt, EDTA-Na₂, Edatamil, Edetato disodium salt dihydrate, Etilenodiaminotetraacetato de disodio dihydrate, Sequestrene Na₂
• Fórmula empírica (notación de Hill): C₁₀H₁₄N₂Na₂O₈ · 2H₂O
• Número de CAS: 6381-92-6
• Peso molecular: 372.24
• Beilstein: 3900609
• Número CE: 205-358-3
• Código UNSPSC: 12352107
• ID de la sustancia en PubChem: 24894586
• NACRES: NA.77

Propiedades

• grado: ACS reagent
• Nivel de calidad: 200
• Agency: suitable for SM 4500 - NH3
• Análisis: 99.0-101.0%
• formulario: solid
• idoneidad de la reacción: reagent type: chelator
• impurezas: ≤0.005% Insoluble matter; ≤0.1% Nitrilotriacetic acid ([HOCOCH₂]₃N)
• pH: 4.0-6.0 (25 °C, 5%)
• mp: 248 °C (dec.) (lit.)
• solubilidad: H₂O: 100 g/L at 20 °C
• trazas de catión: Fe: ≤0.01%; heavy metals: ≤0.005% (by ICP-OES)
• aplicaciones: microbiology
• temp. de almacenamiento: room temp
• cadena SMILES: O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
• Clave InChI: OVBJJZOQPCKUOR-UHFFFAOYSA-L

Descripción

Descripción general

Ethylenediaminetetraacetic acid disodium salt dihydrate or EDTA, is a chelating agent with many applications in molecular biology. EDTA is often used in buffers as a metal ion inhibitor to maintain the stability and integrity of biological samples. EDTA binds to metal cations by forming a stable octahedral complex. Its ability to chelate metal ions makes it extremely useful for blocking metal-dependent enzymatic activities, such as DNases and proteases. Additionally, EDTA is often found in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Aplicación

Antioxidant assays (1) EDTA is also employed in molecular biology techniques, such as DNA extraction, purification, and electrophoresis, where it helps to prevent metal-mediated degradation of nucleic acids. Additionally, EDTA is utilized in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Ethylenediaminetetraacetic acid disodium salt dehydrate has been used as a component:

• of radioimmunoprecipitation assay buffer (RIPA buffer) for preparation of C2C12 mouse skeletal myoblasts whole cell lysate
• of lysis buffer and IgG resin wash and equilibration buffer for the purification of human proteasome regulatory particles (RPs)
• of M-buffer for the isolation of human cardiomyocyte progenitor cells (CMPCs) from fetal heart

Quelante de cationes divalentes. Inhibe las enzimas, como las metaloproteasas, que requieren cationes divalentes para ser activas.

Características y beneficios

American Chemical Society (ACS) grade is suitable for use in standard analytical methods

Otras notas

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Información de seguridad

• Pictogramas: GHS08,GHS07
• Palabra de señalización: Warning
• Frases de peligro: H332,H373
• Consejos de prudencia: P260 - P271 - P304 + P340 + P312 - P314 - P501
• Clasificaciones de peligro: Acute Tox. 4 Inhalation - STOT RE 2 Inhalation
• Órganos de actuación: Respiratory Tract
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)