C3138
Cathepsin D from bovine spleen
lyophilized powder, ≥2.0 units/mg protein
Sinónimos:
CTSD
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About This Item
Número de CAS:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.32
Productos recomendados
origen biológico
bovine spleen
Nivel de calidad
Ensayo
10—70% protein (biuret)
Formulario
lyophilized powder
actividad específica
≥2.0 units/mg protein
mol peso
~45 kDa
fabricante / nombre comercial
Sigma-Aldrich
técnicas
activity assay: suitable
color
dark brown
idoneidad
suitable for molecular biology
Nº de acceso UniProt
aplicaciones
life science and biopharma
temp. de almacenamiento
−20°C
Información sobre el gen
cow ... CTSD(282883)
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Categorías relacionadas
Descripción general
Research area: Cell Signaling
Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.
Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.
Aplicación
Cathepsin D from bovine spleen has been used:
- in in vitro dose-dependent fluorometric activity assays.
- in in vitro myelin oligodendrocyte glycoprotein (MOG) digestion to study the uptake of malondialdehyde (MDA)-modified MOG and its implications in central nervous system autoimmunity.
- for enzymatic digestion of the proteoglycan moiety of the articular cartilage in order to determine its dynamic elastic modulus at two different levels of tissue organization.
- in cathepsin D activity assay.
Acciones bioquímicas o fisiológicas
Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.
Endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts.
Definición de unidad
One unit will produce an increase in A280 of 1.0 per min per mL at pH 3.0 at 37 °C measured as TCA-soluble products using hemoglobin as substrate (1 cm light path).
Forma física
Lyophilized powder containing citrate buffer salts
Inhibidor
Referencia del producto
Descripción
Precios
Producto relacionado
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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