B5283
Anti-Rabbit IgG (γ-chain specific)−Biotin antibody, Mouse monoclonal
clone RG-96, purified from hybridoma cell culture
Sinónimos:
Monoclonal Anti-Rabbit IgG (γ-chain specific)
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.46
Productos recomendados
origen biológico
mouse
conjugado
biotin conjugate
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
secondary antibodies
clon
RG-96, monoclonal
Formulario
buffered aqueous solution
reactividad de especies
rabbit
no debe reaccionar con
goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken
técnicas
direct ELISA: 1:150,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000
western blot (chemiluminescent): 1:200,000-1:400,000
isotipo
IgG1
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
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Descripción general
IgG (immunoglobulin G) antibody is secreted by B cells and is found in blood and extracellular fluids and is enriched in mother′s milk. The variable region of IgG antibody is specific to antigens and is highly conserved.
The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.
Aplicación
Monoclonal Anti-Rabbit IgG (γ-chain specific)-Biotin is suitable for ELISA at a working dilution of 1:150,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000. The antibody was used in β-catenin ELISA at a dilution of 1:2000.
Anti-Rabbit IgG (γ-chain specific)-Biotin antibody, Mouse monoclonal has been used in β-Catenin enzyme immunoassay. It has also been used in immunohistochemistry for examining histology of mammary glands.
Anti-Rabbit IgG (γ-chain specific)-Biotin antibody, Mouse monoclonal has been used in β-Catenin enzyme immunoassay. It has also been used in immunohistochemistry for examining histology of mammary glands.
Acciones bioquímicas o fisiológicas
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Rabbit IgG (γ-chain specific) antibody allows for the binding of various labels such as avidin or streptavidin. IgG antibody has its function similar to IgM antibody in complement system activation.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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