B300-05

Bovine Coronary Artery Endothelial Cells: BCAEC (Cryovial)

• Código UNSPSC: 41106514
• NACRES: NA.81

Propiedades

• origen biológico: bovine coronary artery (USDA-inspected cattle)
• Nivel de calidad: 100
• envase: pkg of 500,000 cells
• fabricante / nombre comercial: Cell Applications, Inc
• modo de crecimiento: Adherent
• cariotipo: 2n = 60
• morfología: Endothelial
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cardiovascular diseases
• temp. de almacenamiento: −196°C

Descripción

Descripción general

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BCAEC have been used to demonstrate that the stress hormone cortisol inhibits NO synthase and Ca2+ mobilization leading to decreased NO release by endothelial cells, resulting in increased arterial contraction and decreased blood flow (Rogers, 2002). Similar action mechanism were determined for cocaine effects on arteries (He, 2005). Additionally it was found that cocaine-induced apoptosis of endothelial cells is mediated by attenuation of NO production, down-regulation of Bcl-2 and translocation of Bac to the mitochondria, followed by release of cytochrome c and activation of caspase 9 and caspase 3 (He, 2000, 2001). BCAEC were also used to determine that diabetes-related vascular dysfunction is mediated by decreased NO production due to direct substrate competition between NO synthase and arginase whose activity is markedly increased in many conditions characterized by vascular dysfunction (Romero, 2008). BCAEC were also used to evaluate novel mesh-like materials for their suitability for biomedical applications (Nair, 2004).

Origen línea celular

Artery

Aplicación

cardiovascular function, angiogenesis, vascular biology, regulation of coronary blood flow, cardiac functions, effects of vaso-dilators, coagulation, fibrinolysis, vessel wall tone, cytokine production

Componentes

Bovine Endothelial Cell Growth Medium containing 10% FBS & 10% DMSO

Nota de preparación

• 3rd passage, >500,000 cells in Bovine Endothelial Cell Growth Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 16 doublings

Rutina de subcultivo

Please refer to the BCAEC Culture Protocol.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable