APPA014

HIV Protease Activity Detection Kit

• Sinónimos: Cut-N-Glow HIV Protease Detection Kit
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• formulario: solution
• Nivel de calidad: 200
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C

Descripción

Aplicación

This kit is specific for the HIV Protease, a retroviral aspartyl protease that is critical to viral life-cycle. The Cut-N-Glow approach involves the introduction of a structural distortion into one of the complementary fragments (GFP 11), through the use of a conditionally stable tether, which serves to constrain the N and C termini of GFP 11 closely in space, thereby, diminishing the mutual affinity of the two fragments and blocking protein self-assembly until the tether is cleaved. The distortion can be reversed upon site-specific proteolysis of the tether, resulting in GFP 11 fragment assembly with GFP 1-10, generating reconstituted, functional GFP. Depending on the tether, the chimeric GFP can serve as a substrate for proteases from the three major classes: serine, cysteine, and aspartic acid.

Idoneidad

This kit contains sufficient reagents for one 96 well plate (96 tests)

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable