A9316
Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat
affinity isolated antibody, buffered aqueous glycerol solution
Sinónimos:
Goat Anti-Mouse IgG (whole molecule)−AP
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About This Item
Productos recomendados
origen biológico
goat
conjugado
alkaline phosphatase conjugate
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
secondary antibodies
clon
polyclonal
formulario
buffered aqueous glycerol solution
técnicas
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
modificación del objetivo postraduccional
unmodified
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Descripción general
Goat polyclonal anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody is reactive with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM. Cross reactivity of the antibody conjugate is determined by ELISA. The conjugate shows no reactivity with rat IgG or rat serum proteins.
Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant immunoglobulin present in human serum. The four classes of IgG are IgG1, IgG2, IgG3, and IgG4.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Aplicación
Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in goat has been used as a secondary antibody in:
- histological studies in heart and liver grafts
- immunohistochemical studies of gangliosides and immunostaining of lymphoid organs
- western blot detection in blood samples
Surfactant Protein A was detected in bronchoalveolar fluid using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at μg/ml in TBS/Tween containing final concentration of 0.5M NaCl.
The level of antibodies specific to P. aeuginosa present in immunized mouse sera was determined by ELISA using alkaline phosphatase-conjugated goat anti-mouse IgG as the secondary antibody at a dilution of 1:1000.
Acciones bioquímicas o fisiológicas
IgG1 deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection.
Otras notas
Antibody adsorbed with rat serum proteins.
Forma física
Solution in 0.05 M Tris, pH 8.0, containing 10 mM glycine, 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide.
Nota de preparación
Adsorbed to reduce background with rat samples.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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