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Merck

A0162

Sigma-Aldrich

Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Goat Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−AP

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

origen biológico

goat

conjugado

alkaline phosphatase conjugate

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

secondary antibodies

clon

polyclonal

formulario

buffered aqueous solution

reactividad de especies

mouse

técnicas

direct ELISA: 1:3,000 using IgG, IgA, IgM
western blot: 1:50 dilution

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

Descripción general

Immunoglobulins are proteins produced by B cells in response to antigen and regulate response to bacteria and viruses. IgG is known to regulate complement fixation and placental transport. IgA has a crucial role in mucosal immunity as it restricts pathogens from entering the mucosal membrane. IgM is the largest antibody having a pentameric structure which modulates polyreactivity and removes apoptotic cells.

Aplicación

Alkaline-phosphatase-conjugated anti-mouse polyvalent immunoglobulin was used to detect antibodies from hybridomas by indirect ELISA. The antibody was incubated in microtiter plates for 1 hour at 37°C and detected using p-nitrophenyl phosphate substrate (Sigma). Alkaline-phosphatase-conjugated anti-mouse polyvalent immunoglobulin was used to detect the reactivity of fungal cytoplasmic protein with proteins from the serum of mice infected with yeast-form Y cells by western blot analysis. The antibody was used at a 1:50 dilution.

Forma física

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Infection and immunity, 70(10), 5462-5470 (2002-09-14)
Mice immunized with heat-inactivated, whole yeast-form cells (Y cells) of Candida albicans developed intense, specific humoral and cell-mediated immune responses. However, they were modestly protected against a lethal challenge by the fungus, and their sera did not confer passive protection
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