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Merck

A7721

Sigma-Aldrich

JumpStart Taq Antibody

Adds hot-start capabilities to any Taq DNA Polymerase

Sinónimos:

Hot start Taq antibody

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About This Item

Número MDL:
Código UNSPSC:
41106300
NACRES:
NA.55

origen biológico

mouse

conjugado

unconjugated

clon

monoclonal

formulario

liquid

Características

hotstart

concentración

1.1 mg/mL

técnicas

PCR: suitable

color

colorless

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Descripción general

JumpStart Taq Antibody provides an efficient method for hot-start PCR. This antibody is a neutralizing monoclonal antibody specific to Taq DNA polymerase. When the antibody is bound to Taq, the enzyme is rendered inactive. At the first denaturation step in thermal cycling, the enzyme-antibody complex dissociates, and the JumpStart Taq Antibody becomes nonfunctional. Once the antibody is denatured, the activity of the Taq DNA polymerase is restored and the enzyme functions normally during the PCR reaction. JumpStart Taq Antibody is commonly used to prevent undesirable amplification products.

Aplicación

JumpStartTaq Antibody is suitable for use in:
  • cytochrome blocked nucleic acid (LNA) probe assay
  • ligation-mediated polymerase chain reaction (LM-PCR)
  • in Real-Time quantitative PCR Analysis of viral titer
  • PCR amplification of complex genomic or cDNA templates
  • to amplify very low-copy-number targets
  • for the PCR amplification of many thermal cycles (>35) and multiple primer pairs in the same reaction tube

Envase

Supplied with dilution buffer.

Componentes

JumpStart Taq Antibody, 1.1μg/1μL
Dilution Buffer provided in 1 mL vials

Cantidad

Two units of Taq DNA polymerase are inactivated by 1 test of JumpStart Taq Antibody.

Otras notas

JumpStart Taq Antibody is for R&D use only.Not for drug, household, or other uses.

View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Información legal

JumpStart is a trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

D J Sharkey et al.
Bio/technology (Nature Publishing Company), 12(5), 506-509 (1994-05-01)
We demonstrate the utility of antibodies to the DNA polymerase from Thermus aquaticus (TaqPol) as thermolabile inhibitors of TaqPol activity. One of the limitations of the polymerase chain reaction (PCR) is the co-amplification of non-specific products caused by TaqPol activity
Enhanced gene targeting of adult and pluripotent stem cells using evolved adeno-associated virus
Gene correction, 169-179 (2014)
Measuring and monitoring apoptosis and drug toxicity in HIV patients by ligation-mediated polymerase chain reaction
Hooker DJ, et al.
Journal of Cellular and Molecular Medicine, 13(5), 948-958 (2009)
Application of real-time and multiplex polymerase chain reaction assays to study leaf blotch epidemics in barley
Fountaine JM, et al.
Phytopathology, 97(3), 297-303 (2007)

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