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Merck

C7559

Sigma-Aldrich

Cloroformo

≥99%, PCR Reagent, contains amylenes as stabilizer

Sinónimos:

Triclorometano, Tricloruro de metilidino

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About This Item

Fórmula empírica (notación de Hill):
CHCl3
Número de CAS:
Peso molecular:
119.38
Beilstein:
1731042
Número CE:
Número MDL:
Código UNSPSC:
12191502
ID de la sustancia en PubChem:
NACRES:
NA.28

grado

PCR Reagent

Nivel de calidad

densidad de vapor

4.1 (vs air)

presión de vapor

160 mmHg ( 20 °C)

Análisis

≥99%

formulario

liquid

contiene

amylenes as stabilizer

envase

vial of 1.5 mL

técnicas

PCR: suitable

color

colorless

índice de refracción

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

densidad

1.492 g/mL at 25 °C (lit.)

actividad extraña

DNase, RNase, none detected

cadena SMILES

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

Clave InChI

HEDRZPFGACZZDS-UHFFFAOYSA-N

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Descripción general

Chloroform is used in pharmaceutical preparations as an anesthetic and as an additive. It is now mainly used as an organic solvent. Chloroform is produced by anthropogenic and natural sources. This abundant halocarbon in the atmosphere is found in ground and surface water sources.

Aplicación

Chloroform has been used in phenol-chloroform extraction to find its ability to purify nucleic acids from blood culture fluids. It has also been used in RNA extraction/isolation and purification.

Pictogramas

Skull and crossbonesHealth hazard

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Órganos de actuación

Central nervous system, Liver,Kidney

Código de clase de almacenamiento

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

does not flash

Punto de inflamabilidad (°C)

does not flash

Equipo de protección personal

Eyeshields, Faceshields, Gloves


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protocolos

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization

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