A7549
Anti-Apoptosis-Inducing Factor antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Sinónimos:
Anti-AIF
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About This Item
Productos recomendados
origen biológico
rabbit
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
IgG fraction of antiserum
tipo de anticuerpo
primary antibodies
clon
polyclonal
Formulario
buffered aqueous solution
mol peso
antigen 57 kDa
reactividad de especies
mouse, human
envase
antibody small pack of 25 μL
técnicas
microarray: suitable
western blot: 1:1,000 using human epitheloid carcinoma HeLa cell extract and mouse brain extract
Nº de acceso UniProt
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... AIFM1(9131)
mouse ... Aifm1(26926)
Descripción general
Apoptosis-Inducing Factor (AIF) is a mitochondrial flavoprotein that can induce apoptosis in isolated nuclei. Studies have reported that AIF can induce the realease of cytochrome c and caspase 9. Bcl-2 is known to inhibit AIF release without affecting its apoptotic functions.
Rabbit Anti-AIF antibody recognizes human and mouse AIF (57kDa). Staining of AIF in immunoblotting is specifically inhibited with the AIF immunizing peptide (human, amino acids 593-613).
Rabbit Anti-AIF antibody recognizes human and mouse AIF (57kDa). Staining of AIF in immunoblotting is specifically inhibited with the AIF immunizing peptide (human, amino acids 593-613).
Apoptosis-inducing factor (AIF) is encoded by the gene mapped to human chromosome X (Xq25-Xq26). It is a 57 kDa mitochondrial flavoprotein. AIF contains two mitochondrial localization sequences and two putative nuclear localization sequences.
Inmunógeno
synthetic peptide corresponding to the C-terminus of human Apoptosis Inducing Factor (AIF), (amino acids 593-613), conjugated to KLH.
Aplicación
Protein lysates from 2CLL, 10 MCl and Jeko-1 cells were analyzed by western blot using anti-AIF as the primary antibody. Cells also underwent intracellular delivery by being transfected with anti-AIF at a final dilution of 1:2500 and then analyzed by FLOW cytometry.
Rabbit Anti-Apoptosis-Inducing Factor (AIF) antibody has been used for immunoblot analysis at 1:1000 and 1:2000 dilutions. The antibody can also be used for microarray applications.
Acciones bioquímicas o fisiológicas
Microinjection of apoptosis-inducing factor (AIF) into the cytoplasm of intact cells induces chromatin condensation, dissipation of the mitochondrial transmembrane potential and exposure of phosphatidylserine in the plasma membrane.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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PARP Inhibitors for Cancer Therapy, 103-117 (2006)
Life with or without AIF
Hangen E, et al.
Trends in Biochemical Sciences, 35(5), 278-287 (2010)
Export of mitochondrial AIF in response to proapoptotic stimuli depends on processing at the intermembrane space
Otera H, et al.
The Embo Journal, 24(7), 1375-1386 (2005)
Toxic proteins released from mitochondria in cell death
Saelens X, et al.
Oncogene, 23(16), 2861-2861 (2004)
Ricardo Parrondo et al.
Molecular cancer, 9, 182-182 (2010-07-14)
NF-kappaB is a transcription factor that promotes inhibition of apoptosis and resistance to chemotherapy. It is commonly believed that inhibition of NF-kappaB activity can increase sensitivity of cancer cells to chemotherapy. However, there is evidence that NF-kappaB activation can sensitize
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