A5168

Adenosine Deaminase from bovine spleen

Type IX, ammonium sulfate suspension, 150-200 units/mg protein

• Sinónimos: Adenosine aminohydrolase
• Número de CAS: 9026-93-1
• Comisión internacional de enzimas: 3.5.4.4 (BRENDA, IUBMB)
• Número CE: 232-817-5
• Número MDL: MFCD00081287
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: bovine spleen
• Nivel de calidad: 200
• tpo: Type IX
• Formulario: ammonium sulfate suspension
• actividad específica: 150-200 units/mg protein
• mol peso: 32.5-33 kDa
• Nº de acceso UniProt: P56658
• actividad extraña: 5′-AMP deaminase ≤0.01%; alkaline phosphatase ≤0.01%; guanase ≤0.01%; nucleoside phosphorylase ≤0.1%
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: cow ... ADA(280712)

Descripción

Descripción general

Adenosine deaminase/ADA is an enzyme, that is mainly present in lymphocytes and monocytes.

Molecular weight: 32.5-33 kDa

pI: 4.85

Aplicación

Adenosine Deaminase from bovine spleen has been used to metabolize adenosine triphosphate (ATP) and adenosine to an inactive form (inosine). It has also been used to as an additive in KRH (Krebs−Ringer solution buffered with Hepes, containing CaCl2) medium in order to remove exogenous adenosine for cyclic adenosine monophosphate (cAMP) assay.

Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Acciones bioquímicas o fisiológicas

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

Adenosine deaminase/ADA can be used as an important marker to identify tuberculous pleural effusion. The activity of ADA is usually high in liver disease, tuberculosis, typhoid, infective mononucleosis and several malignancies.

Definición de unidad

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Forma física

Suspension in 3.2 M (NH₄)₂SO₄, 0.01 M potassium phosphate, pH 6.0

Nota de análisis

Protein determined by biuret.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)