A2765

Azocasein

protease substrate, chromogenic, powder

• Sinónimos: Sulfanilamide-azocasein
• Número de CAS: 102110-74-7
• Número MDL: MFCD00130592
• Código UNSPSC: 12352204
• NACRES: NA.32

Propiedades

• product name: Azocasein, protease substrate
• origen biológico: bovine
• formulario: powder
• técnicas: ligand binding assay: suitable
• solubilidad: water: 5 mg/mL, clear, orange to very deep orange
• ε (coeficiente de extinción): ≥25 at 440 nm in 0.1 M NaOH at 1%
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.

Aplicación

Azocasein has been used as a substrate for determination of protease activity.

Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.

Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.

Ligadura / enlace

View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)