A0349

Anti-l-Afadin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Número MDL: MFCD03455360
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~200 kDa
• reactividad de especies: canine, mouse, rat, human
• envase: antibody small pack of 25 μL
• validación mejorada: independent; Learn more about Antibody Enhanced Validation
• técnicas: immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections.; indirect immunofluorescence: 1:500 using MDCK cells and cultured human HepG2 cells.; microarray: suitable; western blot: 1:2,000 using extract of rat brain
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MLLT4(4301) ; mouse ... Mllt4(17356) ; rat ... Mllt4(26955)

Descripción

Descripción general

Afadin is a multidomain protein that binds F-actin and E-catenin proteins at cell-cell adheren junctions. I-afadin is a ubiquitously expressed splice variant of afadin that contains PDZ and F-actin binding domains at the C-terminal end. I-afadin is homologous to the human AF-6 protein .

Especificidad

Anti-l-Afadin antibody is specific for I-afadin (approximately 200 kDa). It also binds I-afadin in rat, mouse, dog and human.

Inmunógeno

Synthetic peptide sequence corresponding to amino acid residues 1814-1829 of rat afadin with N-terminal cysteine conjugated to maleimide-activated KLH.

Aplicación

Anti-l-Afadin antibody produced in rabbit has been used in:

• western blotting
• immunofluorescence experiments
• immunocytochemistry
• immunohistochemistry

Acciones bioquímicas o fisiológicas

AF-6/Afadin regulates the formation of tight and adherens junctions in the cell and its absence in mice leads to embryonic defects and lethality . Afadin is implicated in primary breast cancer and pancreatic cancer. It downregulates the process of migration and metastasis in cancer cells.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable