A0293

Anti-Human IgG (Fab specific)−Peroxidase antibody produced in goat

affinity isolated antibody

• Sinónimos: Anti Human Fab Antibody, Anti Human Fab Antibody - Anti-Human IgG (Fab specific)-Peroxidase antibody produced in goat
• Número MDL: MFCD00162434
• Código UNSPSC: 12352203
• NACRES: NA.46

Propiedades

• origen biológico: goat
• conjugado: peroxidase conjugate
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: secondary antibodies
• clon: polyclonal
• reactividad de especies: human
• técnicas: direct ELISA: 1:40,000; dot blot: 1:100,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200; western blot (chemiluminescent): 1:100,000
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders,.
Antibody is isolated from anti-human IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. Anti-Human IgG is conjugated to peroxidase and then further purified to remove unconjugated material.

Inmunógeno

Fab fragment of human IgG

Aplicación

Anti-Human IgG (Fab specific)-Peroxidase antibody produced in goat has been used in:

• western blot
• direct enzyme linked immunosorbent assay (ELISA) at 1:40,000 dilution for analysis of yeast cultures genetically modified to produced recombinant Fab fragments.
• immunohistochemistry
• dot blot
• immunohistochemistry (formalin-fixed, paraffin-embedded sections, 1:200)

Anti-Human IgG antibody was used at a 1:10,000 dilution in PBS and incubated for 1 hour at room temperature. Binding in ELISA assays was detected with a O-phenylene-diamine substrate solution (Sigma).

Goat anti-human IgG (Fab specific)-peroxidase antibody can be used for direct ELISA (1:40,000), dot blot and immunohistochemistry (formalin-fixed, paraffin-embedded sections, 1:200) applications.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Nota de preparación

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H317
• Consejos de prudencia: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Clasificaciones de peligro: Skin Sens. 1
• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable