About This Item
Código UNSPSC:
41106514
Productos recomendados
origen biológico
human lymph
Nivel de calidad
formulario
liquid
modo de crecimiento
Adherent
cariotipo
Not specified
morfología
Epithelial
productos
Not specified
receptores
Not specified
técnicas
cell culture | mammalian: suitable
enfermedades relevantes
cancer
Condiciones de envío
dry ice
temp. de almacenamiento
−196°C
Descripción general
COLO 699 N cells, a non-small cell lung cancer cell line is useful in subcutaneous xenografts studies to mimic in vivo model of human lung cancer. It finds applications in stable isotope-based proteome analysis.
Origen línea celular
Human lung cancer
Descripción línea celular
Derived from the pleural fluid of a 57 year old female with lung cancer. Please note: This cell line was found to be indistinguishable from cell lines RPMI 7932 & COLO 738 by STR PCR DNA profiling. At ECACC the STR PCR profile of all stocks of this cell line match the profile of the original source material received at ECACC. However, it is not clear if the cell lines COLO 699N, RPMI 7932 & COLO 738 were originally derived from the same individual as the profiles suggest. The cell lines COLO 699N, RPMI 7932 & COLO 738 were received from the same depositor. If we are able to obtain clarification of the relationship between these cell lines this information will be updated.
Aplicación
COLO 699 N has been used in cytotoxicity studies with Tegaran and 13-methyltetradecanoic acid (13-MTD).
Tumourigenicity studies
Perfil del ADN
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 11,12
D7S820: 11,13
THO1: 9,9.3
TPOX: 8
vWA: 17
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 11,12
D7S820: 11,13
THO1: 9,9.3
TPOX: 8
vWA: 17
Medio de cultivo
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Rutina de subcultivo
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Otras notas
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cláusula de descargo de responsabilidad
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Kathrin Grundner-Culemann et al.
Journal of proteomics, 130, 1-10 (2015-09-13)
Non-small cell lung cancer (NSCLC) cell lines are widely used model systems to study molecular aspects of lung cancer. Comparative and in-depth proteome expression data across many NSCLC cell lines has not been generated yet, but would be of utility
H G Morse et al.
Cancer genetics and cytogenetics, 69(2), 108-112 (1993-09-01)
Melanoma cell lines initiated from metastases excised at the same time from multiple sites in a patient reflect a single clone origin. The similarities of the karyotypes of these lines depend upon the site of excision, the selective survival in
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