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64958

2-Methoxy-2,4-diphenyl-3(2H)-furanone

Name: 2-Methoxy-2,4-diphenyl-3(2<I>H</I>)-furanone
Brand: SIGMA

suitable for fluorescence, ≥98.0% (HPLC)

Sinónimos:

2,4-Diphenyl-2-methoxy-3(2H)-furanone, MDPF

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About This Item

Fórmula empírica (notación de Hill):

C₁₇H₁₄O₃

Número de CAS:

50632-57-0

Peso molecular:

266.29

Beilstein:

1288529

Número MDL:

MFCD00036834

Código UNSPSC:

12352108

ID de la sustancia en PubChem:

329760016

NACRES:

NA.32

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Slide 1 of 1

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Supelco

30965

Furfuryl alcohol

Nivel de calidad

100

Análisis

≥98.0% (HPLC)

formulario

solid

fluorescencia

λ_ex 384 nm; λ_em 472 nm in acetonitrile (after derivatization with hexylamine)

idoneidad

suitable for fluorescence

temp. de almacenamiento

2-8°C

cadena SMILES

COC1(OC=C(C1=O)c2ccccc2)c3ccccc3

InChI

1S/C17H14O3/c1-19-17(14-10-6-3-7-11-14)16(18)15(12-20-17)13-8-4-2-5-9-13/h2-12H,1H3

Clave InChI

BLWINLJDTOJSRU-UHFFFAOYSA-N

Aplicación

2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) is used as a fluorescence reagent to derivatize primary and secondary amines on molecules such as proteins. MDPF derivatized molecules can be detected during analytical and separation procedures including chromatography, electrophoresis and zymography.

Otras notas

Reagent for the derivatization of primary and secondary amines for HPLC; highly fluorescent derivatives are formed; Pre-column derivatization of amines; Fluorescent labeling of proteins before SDS-PAGE

Pictogramas

GHS07

Palabra de señalización

Warning

Frases de peligro

H315,H319,H335

Consejos de prudencia

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Clasificaciones de peligro

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Flow and evaporation cells for the detection of proteins on membranes with the peroxyoxalate chemiluminescent reaction in organic media.

Pablo Castro-Hartmann et al.

Electrophoresis, 25(15), 2501-2505 (2004-08-10)

The high-energy intermediates generated in the reaction of bis(2,4,6-trichlorophenyl)oxalate (TCPO) with H2O2 can excite electronically different fluorophores with a high quantum yield in organic solvents. We have previously applied this peroxyoxalate chemiluminescent reaction to the detection of proteins labeled with

Nonenzymatic chemiluminescent detection and quantitation of total protein on Western and slot blots allowing subsequent immunodetection and sequencing.

F J Alba et al.

Electrophoresis, 18(11), 1960-1966 (1998-01-07)

We have studied the light emission efficiency of proteins labeled with different fluorescent dyes chemically excited by the bis(2,4,6-trichlorophenyl)oxalate (TCPO)-H2O2 reaction. Using this peroxyoxalate chemiluminescence system, the best results were obtained with proteins covalently labeled with 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF). Blotted proteins

Cytofluorometric assay for the determination of thymidine uptake and phosphorylation in living cells.

M Hengstschläger et al.

Cytometry, 14(1), 39-45 (1993-01-01)

Thymidine kinase is a key enzyme for the application of drugs in chemotherapy and for diagnosis. Although of great interest, its regulation during cell cycle and differentiation is difficult to study, as current techniques for isolation of cells in different

Fluorescence of eosinophil leukocyte granules induced by the fluorogenic reagent 2-methoxy-2,4-diphenyl-3 (2H)-furanone.

J C Stockert et al.

Blood cells, 19(2), 423-430 (1993-01-01)

The fluorogenic reagent MDPF forms highly fluorescent products specifically with primary amino groups (e.g., from lysine). Although widely used in analytical biochemistry, MDPF has infrequently been employed in cytochemical techniques for proteins. In this work, we describe the application of

S. Stein

Pept. Neurobiol., 9-9 (1977)

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