506-05F

Human Lung Fibroblasts: HLF, fetal

• Código UNSPSC: 41106514
• NACRES: NA.81

Propiedades

• origen biológico: human lung (normal parenchyma)
• Nivel de calidad: 100
• envase: pkg of 500,000 cells
• fabricante / nombre comercial: Cell Applications, Inc
• modo de crecimiento: Adherent
• cariotipo: 2n = 46
• morfología: fibroblast
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: asthma; tuberculosis; cardiovascular diseases; cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Descripción general

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HLF provide an excellent model system to study many aspects of human pulmonary function and pathophysiology.

HLF have been utilized in a number of research publications, for example to:

• Demonstrate differential gene expression patterns between fetal and adult lung fibroblasts, in particular showing that fetal fibroblasts express higher level of TGF-β receptor interacting protein 1 (TRIP-1) and consequently are less able to contract collagen than adult lung fibroblasts, leading to better healing of acute injuries in very immature lungs compared to higher levels of fibrosis observed in older patients (Navarro, 2009), and additionally discover that TRIP-1 regulates lung fibroblast/myofibroblast trans-differentiation (Nyp, 2014)
• Show that angiotensin II promotes lung fibrosis by activating expression of IL-4, IL-7, and PDGF-D increasing the extracellular matrix formation, and that this process can be blocked by angiotensin receptor inhibitor candesartan (Okada, 2009)
• Determine that phosphorylation of C/EBPβ contributes to lung fibrosis (Buck, 2011)
• Demonstrate that increased tissue rigidity activates toll-like receptor 2 and NF-κB in lung fibroblasts and induces expression of cytokines, CXCL1-3, IL-8 and TNF-α (Zheng, 2011)
• Demonstrate that 2-ethoxyestradiol, a non-estrogenic estradiol metabolite analog, inhibits cell growth and retards the progression of pulmonary fibrosis and hypertension (Tofovic, 2008, 2009)
• Identify the hypoxia-induced ROS/RhoA–ROCK dependent actin reorganization as a mediator of myofibroblast transformation and collagen synthesis in lung fibrosis (Ni, 2013)

Origen línea celular

Lungs

Aplicación

pulmonary function, gene expression, healing and repair process, differentiation, extracellular matrix production, growth factor and cytokine activation, actin reorganization, synthesis of type II collagen, elastin, and proteoglycans, airway remodeling, pharmaceutical development

Componentes

Basal Medium containing 10% FBS & 10% DMSO

Nota de preparación

• 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 12 doublings

Rutina de subcultivo

Please refer to the HLF Culture Protocol.

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable