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Merck

19899

Sigma-Aldrich

CHAPS 100 mM solution

Sinónimos:

CHAPS solution

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About This Item

Fórmula empírica (notación de Hill):
C32H58N2O7S
Número de CAS:
Peso molecular:
614.88
Número MDL:
Código UNSPSC:
12161902
ID de la sustancia en PubChem:
NACRES:
NA.25

descripción

zwitterionic

formulario

liquid

mol peso

614.88 g/mol

concentración

100 mM in H2O

técnicas

protein quantification: suitable

CMC

8 mM

temperatura de transición

cloud point >100 °C

densidad

1.01 g/mL at 20 °C

temp. de almacenamiento

2-8°C

cadena SMILES

C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

InChI

1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1

Clave InChI

UMCMPZBLKLEWAF-RFCNGIAKSA-N

Descripción general

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) is a biocompatible surfactant. It is a derivative of cholic acid comprising a hydrophobic cholesterol-like skeleton possessing three hydroxyl groups and a zwitterionic aliphatic tail.

Aplicación

CHAPS 100 mM solution has been used as a component of lysis buffer for the extraction of proteins from goat endometrial epithelial cells (EECs) and liver tissue.

Acciones bioquímicas o fisiológicas

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) exhibits the properties of both sulfobetaine-type surfactants and bile salts. It is involved in the protein extraction process for proteomic analysis and reconstitution of membrane proteins into lipid vesicles (proteoliposomes). CHAPS is broadly used in biochemistry for membrane protein and receptor isolation and purification. It is conveniently used in pharmaceuticals and medicines due to its biocompatibility, noncharged character, nondenaturing, and disaggregating properties.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves


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