T1438
Todd Hewitt broth
suitable for microbiology, NutriSelect® Plus
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About This Item
Código UNSPSC:
41171607
NACRES:
NA.85
Productos recomendados
esterilidad
non-sterile
Nivel de calidad
formulario
powder
caducidad
limited shelf life, expiry date on the label
fabricante / nombre comercial
NutriSelect® Plus
técnicas
microbiological culture: suitable
pH
7.8±0.2 (25 °C)
aplicaciones
clinical testing
food and beverages
microbiology
temp. de almacenamiento
2-25°C
idoneidad
nonselective for Streptococcus spp.
Categorías relacionadas
Descripción general
Todd Hewitt Broth is used for cultivation of haemolytic streptococci for different serological tests. This medium has been recommended as an alternative type in epidemiologic studies of group A streptococci as well as pathogenic microorganisms. Todd Hewitt Broth medium is very nutritive by the presence of peptic digest of animal tissue and beef heart infusion. Dextrose stimulates haemolysin production. The medium is well buffered by sodium phosphate and sodium carbonate to neutralize the acid produced during dextrose fermentation. This restricts destruction of antigenic streptococcal haemolysin. It is also found that sodium phosphate has a stimulating effect on the pneumococcal growth. Todd Hewitt Broth can be employed as an alternative to serum broth or horse flesh digest broth for the cultivation of streptococci prior to serological typing.
Aplicación
Recommended for the cultivation of group A hemolytic Streptococci used for serological studies.
Todd Hewitt broth has been used for the culture of various microorganisms, such as Bacillus subtilis and Streptococcus pneumoniae.
Componentes
Components (g/L):
Beef Heart Infusion 500.00
Peptic Digest of Animal Tissue 20.00
Dextrose 2.00
Sodium Chloride 2.00
Sodium Phosphate 0.40
Sodium Carbonate 2.50
Beef Heart Infusion 500.00
Peptic Digest of Animal Tissue 20.00
Dextrose 2.00
Sodium Chloride 2.00
Sodium Phosphate 0.40
Sodium Carbonate 2.50
Nota de preparación
Suspend 37 g of Todd Hewitt Broth in 1000 ml of distilled water. Mix well and dispense as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Nota al pie de página
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Información legal
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Faceshields, Gloves, type N95 (US)
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Artículos
Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques
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