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Merck

R1148

Supelco

Rogosa SL agar

suitable for microbiology, NutriSelect® Plus

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About This Item

Código UNSPSC:
41171606
NACRES:
NA.85

esterilidad

non-sterile

Nivel de calidad

100
200

formulario

powder

caducidad

limited shelf life, expiry date on the label

fabricante / nombre comercial

NutriSelect® Plus

técnicas

microbiological culture: suitable

pH final

5.4±0.2 (25 °C)

aplicaciones

bioburden testing
clinical testing
food and beverages

microbiology

temp. de almacenamiento

2-8°C

idoneidad

selective by low pH for Lactobacillus spp.

Aplicación

Used as a selective medium for cultivation of Lactobacilli.

Componentes

Ingredients (g/L)
Tryptose, 10.00
Yeast extract, 5.00
Dextrose, 10.00
Arabinose, 5.00
Saccharose, 5.00
Sodium acetate, 15.00
Ammonium citrate, 2.00
Monopotassium phoshate, 6.00
Magnesium sulphate, 0.57
Manganese sulphate, 0.12
Ferrous sulphate, 0.03
Polysorbate 80, 1.00
Agar, 15.00

Nota de preparación

Suspend 75 g of Rogosa SL Agar in 1000 ml of distilled water. Boil to dissolve the medium completely. Add 1.32 ml of glacial acetic acid (Cat. No. 45726). Mix thoroughly, and distribute into culture tubes or flasks. Heat to 90-100°C for 2-3 minutes. Cool to 45°C for direct inoculation.
DO NOT AUTOCLAVE.

Nota al pie de página

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Información legal

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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A selective medium for the isolation and enumeration of oral and fecal lactobacilli.
M ROGOSA et al.
Journal of bacteriology, 62(1), 132-133 (1951-07-01)

Artículos

Molecular biology-based methods, like PCR, can be used for lactobacilli detection.

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

Today, diverse studies report the benefits of probiotics, such as inhibitory effects on pathogens, aid in the management or prevention of chronic intestinal inflammatory diseases or atopic syndromes, and support to the immune system. Potential beneficial applications abound, researchers continue to evaluate the effictiveness and clarify the mechanisms of action of probiotics.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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