PPB021

SSPE Buffer

pH 7.4, pHast Pack^™, powder

• Sinónimos: 1X SSPE Hybridization buffer, 1X SSPE buffer, SSPE Hybridization buffer, Saline-Sodium Phosphate-EDTA Buffer
• Código UNSPSC: 41105319
• NACRES: NA.25

Propiedades

• Línea del producto: pHast Pack^™
• Nivel de calidad: 100
• Formulario: powder
• pH: 7.4

Descripción

Aplicación

Saline-Sodium Phosphate-EDTA is used as a hybridization buffer in Northern and Southern blotting. It is also used as a wash buffer in DNA-microarray technique.

Características y beneficios

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Save time and minimize effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Envase

Foil pouches

Reconstitución

Contents of one pouch, when dissolved in 1000 mL of ultrapure water, will yield a 1× solution containing 150 mM NaCl, 10 mM NaH₂PO₄, 1 mM EDTA, pH 7.4 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

Almacenamiento y estabilidad

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Otras notas

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Información legal

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1