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M8802

Millipore

Malonate broth

pH 6.7±0.2 (25 °C), NutriSelect® Plus, suitable for microbiology

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About This Item

Código UNSPSC:
41171607
NACRES:
NA.85

esterilidad

non-sterile

Nivel de calidad

200

formulario

powder

fabricante / nombre comercial

NutriSelect® Plus

técnicas

microbe id | utilization test: suitable
microbiological culture: suitable

pH

6.7±0.2 (25 °C)

aplicaciones

food and beverages
water monitoring
microbiology

idoneidad

Klebsiella spp.
Salmonella spp.
nonselective and differential for Enterobacter spp.
nonselective and differential for Escherichia coli
nonselective and differential for coliforms

Descripción general

Malonate broth is recommended for the differentiation of Enterobacter and Escherichia.

Aplicación

Malonate broth has been used to evaluate the utilization of malonate by Enterobacter sakazakii. It has also been used to differentiate between Cronobacter sakazakii and Cronobacter malonaticus isolates.

Componentes

Components (g/L)
Ammonium sulfate, 2.00
Dipotassium phosphate, 0.60
Monopotassium phosphate, 0.40
Sodium chloride, 2.00
Sodium malonate, 3.00
Bromo thymol blue, 0.025

Nota de preparación

Suspend 8 g of Malonate Broth in 1000 ml of distilled water. Dispense and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Avoid the addition of carbon and nitrogen from other sources.

Nota al pie de página

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Información legal

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
BCCG1477
BCCC9735
BCBX0472
BCBW3879
BCBQ6504V

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Carol Iversen et al.
BMC evolutionary biology, 7, 64-64 (2007-04-19)
Enterobacter sakazakii is an opportunistic pathogen that can cause infections such as necrotizing enterocolitis, bacteraemia, meningitis and brain abscess/lesions. When the species was defined in 1980, 15 biogroups were described and it was suggested that these could represent multiple species.
Amy Strydom et al.
Food microbiology, 28(8), 1472-1477 (2011-09-20)
Members of the genus Cronobacter are opportunistic pathogens associated with life-threatening infections in immuno-compromised individuals. Polyphasic analysis has facilitated the classification of the novel genus Cronobacter containing five species. However, since this recent reclassification there are not many identification methods
Ewing, W., et al.
Publ. Hlth. Lab., 15, 153-153 (1957)
MacFaddin, J
Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, 1 (1985)

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