B0126

Bromophenol Blue

Powder

• Sinónimos: 3′,3′′,5′,5′′-Tetrabromophenolsulfonephthalein, Bromophenol Blue (BPB), Bromphenol Blue Sultone Form
• Fórmula empírica (notación de Hill): C₁₉H₁₀Br₄O₅S
• Número de CAS: 115-39-9
• Peso molecular: 669.96
• Beilstein: 61698
• Número CE: 204-086-2
• Número MDL: MFCD00005875
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 24891504
• NACRES: NA.47

Propiedades

• Nombre del producto: Bromophenol Blue, titration: suitable
• Agency: suitable for SM 2310; suitable for SM 2320
• Nivel de calidad: 200
• Formulario: powder
• técnicas: titration: suitable
• color: faint beige to very dark beige; faint orange to very dark orange
• visual transition interval: 3.0-4.6, yellow-green to blue
• mp: 273 °C
• solubilidad: methanol: 10 mg/mL
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: room temp
• cadena SMILES: Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4
• InChI: 1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H
• Clave InChI: UDSAIICHUKSCKT-UHFFFAOYSA-N

Descripción

Descripción general

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein. Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Aplicación

Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)