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114391

Sigma-Aldrich

Bromophenol Blue

ACS reagent

Sinónimos:

3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form

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About This Item

Fórmula empírica (notación de Hill):
C19H10Br4O5S
Número de CAS:
115-39-9
Peso molecular:
669.96
Beilstein:
61698
Número CE:
204-086-2
Número MDL:
MFCD00005875
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
24847169
NACRES:
NA.47

grado

ACS reagent

Nivel de calidad

200

Formulario

powder

técnicas

titration: suitable

visual transition interval

3.0-4.6, yellow to blue

mp

273 °C (lit.)

solubilidad

ethanol: 0.1%

aplicaciones

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

room temp

cadena SMILES

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

Clave InChI

UDSAIICHUKSCKT-UHFFFAOYSA-N

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Descripción general

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Aplicación

Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
BCCG4635
BCCG0495
BCCD6533
SHBL2679
SHBL1423

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