28514

Methylene Blue hydrate

≥95% (calc. to the dried substance)

• Sinónimos: Basic Blue 9 monohydrate ; Methylene Blue (hydrate)
• Fórmula empírica (notación de Hill): C₁₆H₁₈ClN₃S · xH₂O
• Número de CAS: 122965-43-9
• Peso molecular: 319.85 (anhydrous basis)
• Beilstein: 3599847
• Número CE: 200-515-2
• Número MDL: MFCD00150006
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 329753217
• NACRES: MA.02

Propiedades

• Nivel de calidad: 200
• Análisis: ≥95% (calc. to the dried substance)
• formulario: powder
• impurezas: ≤1% insoluble in ethanol
• pérdida: ≤22% loss on drying, 105 °C
• color: very dark green
• solubilidad: ethanol: insoluble ≤1%
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: room temp
• cadena SMILES: O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
• Clave InChI: WQVSELLRAGBDLX-UHFFFAOYSA-M

Descripción

Descripción general

Methylene blue is bis(dimethylamino)phenothiazine hydrochloride (tetramethylthionine),
C₁₆H₁₈N₃SCl. It is a major basic histological dye in pharmacology and microbiology. This heterocyclic aromatic chemical appears as a dark green solid powder, changing to blue in an aqueous solution. Methylene blue is tested for histological and and bacteriological staining, and as a constituent of Wright’s blood stain.

Aplicación

Methylene blue is widely used in several routine staining applications including Mallory’s phloxine-methylene blue on paraffin sections of tissue fixed in Zenker’s fluid for nuclear staining and bacterial staining in milk. It is also suitable for manufacturing the polychrome methylene blue used in the preparation of Romanowsky stains.Methylene Blue hydrate has also been used:

• in the preparation of methylene blue (MB) solution
• to stain zymograms -to label the injection site in the tissue slices
• to pre-treat mice via oral gavage to study its effects on noise-induced hearing loss

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H302
• Consejos de prudencia: P264 - P270 - P301 + P312 - P501
• Clasificaciones de peligro: Acute Tox. 4 Oral
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Gloves