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11427598910

Roche

Biotin-16-ddUTP

≥98% (HPLC), solution, 1 mM, suitable for hybridization

Sinónimos:

Bio-16-ddUTP tetralithium salt, 5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′,3′-dideoxyuridine 5′-triphosphate

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About This Item

Fórmula empírica (notación de Hill):
C32H52N7O17P3S
Número de CAS:
188755-42-2
Peso molecular:
931.78
Código UNSPSC:
41116100

Nivel de calidad

100

Ensayo

≥98% (HPLC)

Formulario

solution

mol peso

955.5 (biotin-16-ddUTP-Li4)

envase

pkg of 25 μL (25 nmol; 1mM)

fabricante / nombre comercial

Roche

concentración

1 mM

técnicas

hybridization: suitable

temp. de almacenamiento

−20°C

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Descripción general

Biotin-16-ddUTP, tetralithium salt; 1mM

Aplicación

  • Biotin-16-ddUTP is used as a substrate for: Terminal Transferase
  • DNA polymerase I (Holoenzyme and Klenow fragment)
  • T4 DNA polymerase
  • Taq DNA polymerase and reverse transcriptase (e.g.,Transcriptor).
Biotin-16-ddUTP is preferentially used for 3′-end labeling of oligonucleotides with Terminal Transferase, recombinant and labeling DNA.
  • The biotin-labeled oligonucleotide can be used as a hybridization probe for: DNA and RNA transfers
  • Colony and plaque screening
  • In situ hybridization
In addition to common hybridization techniques, biotin-labeled oligonucleotides are specifically useful for screening expression libraries for sequence-specific DNA-binding proteins, for example, transcription factors.
The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection.
Oligonucleotides are enzymatically labeled at their 3′ end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine- triphosphate (biotin-ddUTP).
It has been used in the biotinylation of in vitro transcribed tmRNA during surface plasmon resonance (SPR) technique. It has been used in the standard nick translation reaction for tumor DNA labelling during comparative genomic hybridization.

Características y beneficios

Biotin is bound to dideoxyuridine triphosphate via an amide linkage.

Calidad

Typical analysis: At least 85% Biotin-16-ddUTP (HPLC, area%).

Otras notas

For life science research only. Not for use in diagnostic procedures.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

does not flash

Punto de inflamabilidad (°C)

does not flash

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Certificados de análisis (COA)

Lot/Batch Number
74115521
27782224
27782223
27782222
27782221

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van de Vliet M S, et al.
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British journal of haematology, 122(2), 193-201 (2003-07-09)
Chromosomal abnormalities, such as 13q deletions, are emerging as important prognostic factors in multiple myeloma. Fluorescence in situ hybridization (FISH) using specific DNA probes is the technique most widely used for the determination of genomic aberrations in this disease. The
Microarray-based method for detection of unknown genetic modifications.
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Cisplatin is a chemotherapy drug that kills cancer cells by damaging their DNA. In human cells, this damage is repaired primarily by nucleotide excision repair. While cisplatin is generally effective, many cancers exhibit initial or acquired resistance to it. Here

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