PS1019

PhosphoDetect Anti-JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵) Rabbit pAb

liquid, Calbiochem^®

• Sinónimos: Anti-c-Jun N-terminal Kinase 1&2, Phospho-Specific (Thr/Tyr ^183/185), Anti-c-Jun N-terminal Kinase 1&2, Phospho-Specific (Thr/Tyr^183/185)
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: liquid
• contiene: ≤0.1% sodium azide as preservative
• reactividad de especies: mouse, rat, human
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• isotipo: IgG
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: phosphorylation (pThr183/pTyr185)
• Información sobre el gen: human ... MAPK8(5599)

Descripción

Descripción general

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1 and the ~55 kDa JNK2 proteins phosphorylated at Thr¹⁸³ and Tyr¹⁸⁵.

Recognizes the ~49 kDa JNK1 and the ~55 kDa JNK2 phosphorylated at Thr¹⁸³ and Tyr¹⁸⁵ in uv-treated HEK293 cells.

This PhosphoDetect Anti-JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵) Rabbit pAb is validated for use in Immunoblotting for the detection of JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵).

Inmunógeno

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human JNK1 and JNK2

Aplicación

Immunoblotting (1:1000)

Advertencia

Toxicity: Standard Handling (A)

Forma física

In Dulbecco′s PBS (without Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.2-7.4.

Reconstitución

Following initial thaw, aliquot and freeze (-20°C).

Nota de análisis

Positive Control
UV-treated HEK293 cells or sorbitol-treated PC12 cells

Otras notas

Choudhury, B.K., et al. 2002. J. Immunol.169, 5955.
Barr, R.K., et al. 2001. Int. J. Biochem. Cell. Biol.33, 1047.
Dong, C., et al. 2001. J. Clin. Immunol.21, 253.
Janulis, M., et al. 2001. Mol. Cell. Biol.21, 2235.
Pearce, A.K., et al. 2001. Trends Cell Biol.11, 426.
Tong, T., et al. 2001. Exp. Cell Res.269, 64.
Cobb, M.H., et al. 1999. Prog. Biophys. Mol. Biol.71, 479.
Basu, S., et al. 1998. Oncogene17, 3277.

The immunogen sequence is highly conserved among many species, including mouse, rat, chicken, nematode, and Drosophila, but cross-reactivity has not been determined. The immunogen sequence is also conserved in JNK3, but reactivity has not been detected. Antibody should be titrated for optimal results in individual systems.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1