MABT856

Anti-von Willebrand factor (vWF) Antibody, clone IIIE2.34

clone IIIE2.34, from mouse

• Sinónimos: vWF
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: IIIE2.34, monoclonal
• reactividad de especies: human
• envase: antibody small pack of 25 μg
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_000543
• Nº de acceso UniProt: P04275
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... VWF(7450)

Descripción

Descripción general

von Willebrand factor (UniProt: P04275; also known as vWF) is encoded by the VWF (also known as F8VWF) gene (Gene ID:7450) in human. vWF is a multimeric plasma glycoprotein that is synthesized by endothelial cells and plays an important in the maintenance of hemostasis. It is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off in the mature form, which is then cleaved into von Willebrand antigen 2 (aa 23-763) and vWF (aa 764-2813). vWF is known to promote adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. vWF also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. Mutations in VWF gene are linked to von Willebrand diseases 1-3 that are common hemorrhagic disorders caused by impaired platelet aggregation. Two isoforms of vWF have been described that are produced by alternative splicing.

Especificidad

Clone IIIE2.34 is a mouse monoclonal antibody that detects human von Willebrand factor. It targets an epitope with in the VWFD3 domain.

Inmunógeno

Protease fragment of VWF binding to heparin sepharose from human plasma.

Aplicación

Anti-von Willebrand factor, clone IIIE2.34, Cat. No. MABT856, is a mouse monoclonal antibody that detects human von Willebrand factor and has been tested for use in Immunocytochemistry and Western blotting.

Research Category
Cell Structure

Western Blotting Analysis: 1 µg/mL from a representative lot detected von Willebrand factor (vWF) in lysates from Human umbilical vein endothelial cells (HUVEC).

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected von Willebrand factor (vWF) in Human umbilical vein endothelial cells (HUVEC).

Calidad

Evaluated by Western Blotting in human platelets cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected von Willebrand factor (vWF) in human platelets cell lysates.

Descripción de destino

~270 kDa observed, 309.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Protein A purified

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable