MABS827

Anti-ERK1/2 Antibody, clone 16A6.1

clone 16A6.1, from mouse

• Sinónimos: EC: 2.7.11.24, MAP kinase 3, MAPK3, ERT2, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, MNK1, Microtubule-associated protein 2 kinase, p44-ERK1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 16A6.1, monoclonal
• reactividad de especies: rat, human, mouse
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG2bκ
• Nº de acceso NCBI: NP_059043
• Nº de acceso UniProt: P21708
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MAPK3(5595) ; mouse ... Mapk3(26417) ; rat ... Mapk3(50689)

Descripción

Descripción general

Mitogen-activated protein kinase 3 (UniProt: P21708; also known as EC: 2.7.11.24, MAP kinase 3, MAPK3, ERT2, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, MNK1, Microtubule-associated protein 2 kinase, p44-ERK1) is encoded by the mapk3 (also known as Erk1, Prkm3) gene (Gene ID: 50689) in rat. ERK1 (a.k.a. MAPK 3) and ERK2 (a.k.a. MAPK 1 or MAPK 2) are related serine/threonine kinases of the Ras-Raf-MEK-ERK signaling pathway. ERK1/2 are activated by MEK1/2-catalyzed phosphorylation at their conserved Thr-Glu-Tyr (TEY) dual phosphorylation motif, first at the Tyr residue (human/rat ERK1 Tyr204/Tyr203, human/rat ERK2 Tyr187/Tyr183) and then at the Thr residue (human/rat ERK1 Thr202/Thr201, human/rat ERK2 Thr185/Thr181). ERK1/2 are proline-directed kinases that preferentially catalyze the phosphorylation of Pro-Xxx-Ser/Thr-Pro sequence motif in hundreds of cytoplasmic and nuclear substrates, including transcription factors such as Ets, Elk, and c-Fos. Besides this primary structure requirement, many ERK1/2 substrates possess a D-docking site and/or an F-docking site. A variety of scaffold proteins, including KSR1/2, IQGAP1, MP1, and -Arrestin1/2, also participate in the regulation of ERK1/2 MAP kinase cascade. Ras-Raf-MEK-ERK signaling activity is upregulated in about one-third of all human cancers, and targeted inhibition against components of this signaling pathway represents a popular anticancer strategy.

Especificidad

Clone 16A6.1 detects both ERK1 and ERK2 in human, mouse, and rat. This clone targets a sequence corresponding to 21 amino acids in the C-terminal region.

Inmunógeno

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of rat ERK1.

Aplicación

Detect ERK1/2 using this mouse monoclonal Anti-ERK1/2 Antibody, clone 16A6.1, Cat. No. MABS827. It is used in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250-1:1,000 dilution from a representative lot detected ERK1/2 in human testis, human cerebellum, rat cerebral cortex, and mouse small intestine tissues.

Research Category
Signaling

Calidad

Evaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ERK1/2 in 10 µg of RAW264.7 cell lysate.

Descripción de destino

~42 kDa observed; 43.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Ligadura / enlace

Replaces: 05-1152

Forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1