MABN712
Anti-CCL2 Antibody, clone 2D8
clone 2D8, from mouse
Sinónimos:
C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
2D8, monoclonal
reactividad de especies
monkey, mouse, rat, human
técnicas
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotipo
IgG1
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... CCL2(6347)
Descripción general
CCL2, Chemokine (C-C motif) ligand 2, or alternatively known as Monocyte chemotactic protein 1 (MCP-1), Monocyte chemotactic and activating factor (MCAF), Monocyte chemoattractant protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, HC11, and C-C motif chemokine 2 is encoded by the human gene named CCL2/MCP1/SCYA2. CCL2 is a chemokine produced by endothelial cells that attracts memory T cells, monocytes, dendritic cells, and basophils but not neutrophils or eosinophils to sites of inflammation produced by either tissue inquiry or infection. CCL2 also bolsters monocytes anti-tumor activity, and because it is chemotactic for monocytes, CCL2 has been implicated in inflammatory diseases where monocytic invasions have occurred such as in psoriasis, rheumatoid arthritis and atherosclerosis. CCL2 is a bifunctional molecule as well; the whole molecule is a basophil activator but removal of the N-terminal portion converts the molecule into an exclusive eosinophil chemoattractant. CCL2 can also be found expressed at the sites of tooth eruption and bone degradation and is expressed by both osteoclasts and osteoblasts. CCL2 is also expressed in neurons, astrocytes and microglia and its role in neuroinflammation is established. CCL2 expression in glial and neurons is increased in epilepsy, brain ischemia, Alzheimer’s disease, brain injury and experimental autoimmune encephalomyelitis. Finally, CCL2 plays a role in vascular complications associated with type II diabetes and in the production of amylin which contributes to insulin resistance in obesity, which is also associated with type II diabetes. EMD-Millipore’s Anti-CCL2 mouse monoclonal antibody has been tested in western blot against recombinant protein and cell lysates from A549, HeLa, Raw264.7, L1210, C6, and COS7 cell lines.
Inmunógeno
Purified recombinant fragment of human CCL2 expressed in E. Coli.
Aplicación
Detect Monocyte Chemotactic Protein using this mouse monoclonal antibody, Anti-CCL2 Antibody, clone 2D8 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected CCL2 in HeLa cells.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CCL2 in human liver cancer tissue.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CCL2 in A549 cells.
Optimal working dilutions must be determined by end user.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CCL2 in human liver cancer tissue.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CCL2 in A549 cells.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Calidad
Evaluated by Western Blotting in A549, HeLa, Raw264.7, L1210, C6 and COS-7 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CCL2 in A549, HeLa, Raw264.7, L1210, C6 and COS-7 cell lysates
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CCL2 in A549, HeLa, Raw264.7, L1210, C6 and COS-7 cell lysates
Descripción de destino
~14 kDa observed. Uncharacterized bands may appear in some lysate(s).
Forma física
Format: Purified
Purified mouse monoclonal in PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.
Almacenamiento y estabilidad
Stable for 1 year at 2-8°C from date of receipt.
Nota de análisis
Control
A549, HeLa, Raw264.7, L1210, C6 and COS-7 cell lysates
A549, HeLa, Raw264.7, L1210, C6 and COS-7 cell lysates
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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