MABN2406

Anti-Tau Antibody, clone TNT-2

clone TNT-2, from mouse

• Sinónimos: Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: TNT-2, monoclonal
• reactividad de especies: rat, human, mouse
• técnicas: ELISA: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_058519
• Nº de acceso UniProt: P10636
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MAPT(4137) ; mouse ... Mapt(17762) ; rat ... Mapt(29477)

Descripción

Descripción general

Microtubule-associated protein tau (UniProt: P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (MAPTL, MTBT1, TAU) gene (Gene ID: 4137) in human. Tau proteins are mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components. Normally, Tau promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The localization of Tau protein in the Alzheimer s disease (AD) brain is markedly abnormal and may contribute to neuronal dysfunction. In AD, normal soluble cytoskeletal elements, such as Tau and neurofilaments, are transformed into insoluble paired helical filaments (PHFs). This is linked to the post-translational change in Tau, primarily its hyperphosphorylation by a number of protein kinases. In its non-phosphorylated state, Tau promotes rapid and extensive microtubule (MT) polymerization. A significant consequence of Tau hyperphosphorylation in AD is a reduction in its ability to bind microtubules and promote microtubule assembly. Hyperphosphorylated Tau may contribute to a destabilized microtubule network, impaired axonal transport, and ultimately result in NFT formation and neuronal death.

Especificidad

Clone TNT-2 specirfically detects tau in human, mouse, and rat brain. It targets a sequence of 17 amino acids from the N-terminal region that is common to all isoforms (1-6) of tau.

Inmunógeno

KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal region of human Tau.

Aplicación

Anti-Tau Antibody, clone TNT-2, Cat. No. MABN2406, is a highly specific mouse monoclonal antibody that targets Tau and has been tested in ELISA, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: 0.02 µg/mL from a representative lot detected Tau in 10 µg of mouse entorhinal cortex.

Immunohistochemistry Analysis: A representative lot detected Tau in Immunohistochemistry applications (Combs, B., et. al. (2016). Neurobiol Dis. 94:18-31).

ELISA Analysis: A representative lot detected Tau in ELISA applications (Combs, B., et. al. (2016). Neurobiol Dis. 94:18-31).

Western Blotting Analysis: A representative lot detected Tau in Western Blotting applications (Combs, B., et. al. (2016). Neurobiol Dis. 94:18-31).

Immunofluorescence Analysis: A representative lot detected Tau in Immunofluorescence applications (Combs, B., et. al. (2016). Neurobiol Dis. 94:18-31).

Calidad

Evaluated by Western Blotting in rat entorhinal cortex.

Western Blotting Analysis: 0.02 µg/mL of this antibody detected Tau in 10 µg of rat entorhinal cortex lysate.

Descripción de destino

~55 kda Observed. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable