MAB3420A4

Anti-Tau-1 Antibody, clone PC1C6, Alexa Fluor^™ 488 Conjugate

clone PC1C6, from mouse, ALEXA FLUOR^™ 488

• Sinónimos: Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• conjugado: ALEXA FLUOR^™ 488
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: PC1C6, monoclonal
• reactividad de especies: mouse, rat
• reactividad de especies (predicha por homología): bovine (immunogen homology)
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable
• isotipo: IgG2a
• Nº de acceso NCBI: NP_776388
• Nº de acceso UniProt: P10636
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Mapt(17762) , Mapt(281296) ; rat ... Mapt(29477)

Descripción

Descripción general

Tau, a microtubulebinding protein which serves to stabilize microtubules in growing axons, is found to be hyperphosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by glycogen synthase kinase 3 (GSK 3). Cellular and subcellular localization: In situ, anti-tau-1 has a stringent specificity for the axons of neurons. The antibody does not stain the cell bodies or dendrites of neurons, nor does it stain any other cell type (4). However, this in vivo intracellular specificity is not maintained in culture: anti-tau-1 stains the axon, cell bodies, and dendrites of rat hippocampal neurons grown in culture (5). The specificity of anti-tau-1 was originally thought to represent the restricted expression of tau to axons. Later studies revealed that this specificity is dependant on the state of phosphorylation. In dephosphorylated samples (samples treated with alkaline phosphatase) anti-tau-1 stains astrocytes, perineuronal glial cells, and the axons, cell bodies and dendrites of neurons, while in untreated samples, anti-tau-1 stains only axons (6). (The epitope recognized by anti-tau-1 is probably at or near a phosphorylated site.)

Especificidad

This antibody binds to all known electrophoretic species of tau in human, rat, and bovine brain (one-dimensional SDS-PAGE).

Inmunógeno

Purified denatured bovine microtubule associated proteins.

Aplicación

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

This Anti-Tau-1 Antibody, clone PC1C6, Alexa Fluor 488 Conjugate is validated for use in IC, IH for the detection of Tau-1.

Calidad

Evaluated by Immunohistochemistry in adult mouse brain tissue.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Tau-1 in adult mouse brain tissue.

Descripción de destino

79 kDa calculated. Uniprot describes isoforms ranging from 33 to 81 kDa

Forma física

Protein A purified

Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Almacenamiento y estabilidad

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Nota de análisis

Control
Adult mouse brain tissue

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

ALEXA FLUOR is a trademark of Life Technologies

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable