MABN1727

Anti-Beta-IV spectrin Antibody, clone N393/76

clone N393/76, from mouse

• Sinónimos: Spectrin beta chain, non-erythrocytic 4, Spectrin, non-erythroid beta chain 3
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: N393/76, monoclonal
• reactividad de especies: human, rat
• reactividad de especies (predicha por homología): mouse (based on 100% sequence homology)
• técnicas: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotipo: IgG2bκ
• Nº de acceso NCBI: NP_066022
• Nº de acceso UniProt: Q9H254
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... SPTBN1(6711)

Descripción

Descripción general

Spectrin beta chain, non-erythrocytic 4 (UniProt: Q9H254; also known as Beta-IV spectrin, Spectrin, non-erythroid beta chain 3) is encoded by the SPTBN4 (also known as KIAA1642, SPTBN3) gene (Gene ID: 57731) in human. Beta-IV Spectrin belongs to the spectrin family of proteins and is abundantly expressed in brain and pancreatic islets. It belongs to the spectrin family of proteins and contains two CH (calponin-homology) domains, one PH domain, and 18 spectrin repeats. It has been isolated as an interactor of the receptor tyrosine phosphatase ICA512. Spectrin molecule is assembled as a tetramer of two alpha and two beta subunits. A tight association between the amino-terminal domain of an alpha subunit and the carboxyl-terminal domain of a beta subunit forms a heterodimer. In brain, beta-IV Spectrin co-localizes with ankyrin-G 480/270-kDa at initial segments and nodes of Ranvier of myelinated neurons. In brain its highest expression is observed in the cell bodies of large myelinated neurons. It is not detected in non-neuronal cells, including glial and endothelial cells.Beta-IV Spectrin along with ankyrin-G is required for the anchoring of voltage-gated Na+ channels and for cell adhesion molecule anchoring to the actin cytoskeleton. (Ref.: Berghs, S et al. (2000). J. Cell Biol. 151(5): 985-1002).

Especificidad

Clone N393/76 specifically detects human and rat beta-IV Spectrin by immunohistochemistry. it targets a sequence within 192 amino acids in the C-terminal half of human beta-IV Spectrin.

Inmunógeno

A recombinant fusion fragment corresponding to 192 amino acids from the C-terminal half of human beta-IV spectrin.

Aplicación

Anti-Beta-IV spectrin Antibody, clone N393/76, Cat. No. MABN1727, is a highly specific mouse monoclonal antibody that targets beta-IV spectrin and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected beta-IV spectrin in human pancreas, human cervical cancer, and human uterus tissue.

Western Blotting Analysis: A representative lot detected beta-IV spectrin in COS cells transiently transfected with Myc-tagged beta 4-spectrin.

Immunohistochemistry Analysis: A representative lot detected beta-IV spectrin in rat cerebral cortex and hippocampus tissues.

Research Category
Neuroscience

Calidad

Evaluated by Immunohistochemistry in human cerebellum tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected beta-IV spectrin in human cerebellum tissue.

Descripción de destino

288.98 kDa calculated.

Forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1