MABF841

Anti-FCGR2A/C Antibody, clone 11B6

clone 11B6, from mouse

• Sinónimos: Low affinity immunoglobulin gamma Fc region receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-b, Low affinity immunoglobulin gamma Fc region receptor II-c, IgG Fc receptor II-a, IgG Fc receptor II-b, IgG Fc receptor II-c, CDw32, Fc-g
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 11B6, monoclonal
• reactividad de especies: human
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001129691.1; NP_963857.3
• Nº de acceso UniProt: P12318; P31995
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... FCGR2A(2212) , FCGR2B(2213)

Descripción

Descripción general

FcγRI/CD64, FcγRII/CD32, FcγRIII/CD16, and FcγRIV/CD16-2 represent the four known classes of IgG Fc receptors (FcγRs). FcγRII, FcγRIII, and FcγRIV are low-affinity receptors for monomeric IgG, whereas FcγRI is the only high-affinity FcγR. FcγRs play important roles in inflammatory cell activation, clearance, presentation of Ag, and maintenance of IgG homeostasis. In addition to binding immune complex (IC), FcγRs have been shown to bind non-IgG ligands. For example, FcγRII is shown to bind oxLDL, while FcγRIII binding to an E. coli component is reported to negatively regulate the function of macrophage receptor with collagenous structure (MARCO). FcyRII activation is also reported to transduce a negative regulatory signal against CD38 cross-linking-induced proliferation of resting mature B cells. Low affinity immunoglobulin gamma Fc region receptor II-a/b/c (IgG Fc receptor II-a/-b/-c, CDw32, Fc-gamma RII-a/-b/-c, Fc-gamma-RIIa/b/c, FcRII-a/-b/-c, CD32) are encoded by the FCGR2A/B/C (CD32, FCG2, IGFR2) genes in human (UniProt P12318, P31994, P31995; Gene ID 2212, 2213, 9103). The FcγRIIs encoded by the three genes are highly conserved in their extracellular portion, with significant variation in the transmembrane and intracellular regions. All three types of transcripts alternative splicing to generate additional isoforms.

Especificidad

Clone 11B6 recognizes human FcγRIIa and FcγRIIc, but not FcγRIIb, by targeting a conserved cytoplasmic domain sequence in human FcγRIIa and FcγRIIc.

Inmunógeno

Epitope: Cytoplasmic domain.

Linear peptide corresponding to a cytoplasmic domain sequence identical in human FcγRIIa and FcγRIIc.

Aplicación

Anti-FCGR2A/C Antibody, clone 11B6 is an antibody against FCGR2A/C for use in Immunohistochemistry (Paraffin), Immunocytochemistry, Western Blotting.

Immunocytochemistry Analysis: A representative lot detected FcγRIIc immunoreactivity in paraformaldehyde-fixed, Triton X-100-permeabilized human B cell lines carrying the FCGR2C-ORF allele, but not B cell lines with homozygous FCGR2C-STP alleles (Li, X., et al. (2013). Sci. Transl. Med. 5(216):216ra175).
Western Blotting Analysis: A representative lot detected recombinant FcγRIIc cytoplasmic (CY) domain GST fusion, but not FcγRIIb CY domain GST fusion (Li, X., et al. (2013). Sci. Transl. Med. 5(216):216ra175).

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

Calidad

Evaluated by Immunohistochemistry in human bone marrow tissue.

Immunohistochemistry Analysis: An 1:50 dilution of this antibody detected FCGR2A/C in human bone marrow tissue.

Descripción de destino

30.89 kDa calculated.

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable