MABF2819

Anti-Complement C4B Antibody, clone 16D2

• Sinónimos: C4, C4B, Complement C4-B
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rat
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 16D2, monoclonal
• mol peso: calculated mol wt 193 kDa
• purificado por: using protein G
• reactividad de especies: mouse
• envase: antibody small pack of 100
• técnicas: ELISA: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG2aκ
• secuencia del epítopo: Unknown
• Nº de acceso Protein ID: NP_033910
• Nº de acceso UniProt: P01029
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: mouse ... C4b(12268)

Descripción

Especificidad

Clone 16D2 is a rat monoclonal antibody that detects mouse Complement C4B.

Inmunógeno

C57BL/6 thymocytes incubated with saturating amounts of a rat anti-Thy-1 monoclonal antibody and C57BL/6 mouse serum

Aplicación

Quality Control Testing

Evaluated by Immunohistochemistry in Mouse spleen tissue sections.

Immunohistochemistry Applications: A representative lot detected Mouse C4B in frozen Mouse spleen tissue sections.

Tested Applications

ELISA Analysis: A representative lot detected Complement C4B in ELISA applications (Yilmaz, M., et. al. (2021). Nat Neurosci. 24(2); 214-224).

Immunohistochemistry Applications: A representative lot detected Complement C4B in Immunohistochemistry applications (Kremmer, E., et al. (1990). Hybridoma. 9(4): 309-17).

Immunofluorescence Analysis: A representative lot detected Complement C4B in Immunofluorescence applications (Simoni, L., et. al. (2020). Cell Rep. 33(5): 108330).

Western Blotting Analysis: A representative lot detected Complement C4B in Western Blotting applications (Kremmer, E., et al. (1990). Hybridoma. 9(4):309-17).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Complement C4-B (UniProt: P01029; also known as C4B) is encoded by the C4b (also known as C4) gene (Gene ID: 12268) in murine species. The complement system provides innate defense against microbial infection and is a complement to antibody mediated immunity. It consists of about thirty-five interacting plasma and membrane associated proteins that contribute to host-defense. It contains several soluble factors that prevent spontaneous complement activation from occurring in solution, as well as several regulatory membrane-associated proteins that protect host cells from accidental complement attack. Complement C4 is a key component of the complement system and is one of chief constituents of innate immunity for immediate recognition and elimination of invading microbes. It plays an essential role for the functions of both classical and lectin complement pathways. It is a disulfide-bonded three-chain glycoprotein, consisting of an -chain (95 kDa), a β-chain (75 kDa), and a -chain (30 kDa). During complement activation, the -chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the β- and -chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase. Dysregulation of classic and lectin complement pathways where complement C4 plays a vital role can lead to complement-mediated autoimmune diseases. (Ref.: Wang, H., and Liu, M. (2021). Front. Immunol. 12; Article 694928; Kremmer, E., et al. (1990). Hybridoma. 9(4); 309-317).

Forma física

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitución

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommended storage: +2°C to +8°C.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable