MABF2128

Anti-Properdin Antibody, clone 6E11A4

clone 6E11A4, from mouse

• Sinónimos: Complement factor P
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propiedades

• origen biológico: mouse
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 6E11A4, monoclonal
• reactividad de especies: baboon, rabbit, canine, human
• envase: antibody small pack of 25 μg
• técnicas: ELISA: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001138724
• Nº de acceso UniProt: P27918
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CFP(5199)

Descripción

Descripción general

Properdin (UniProt: P27918; also known as Complement factor P) is encoded by the CEP (also known as PFC) gene (Gene ID: 5199) in human. (ABF185: Properdin (UniProt P27918; also known as Complement factor P) is encoded by the CFP (also known as PFC, PFD) gene (Gene ID 5199) in human. Properdin is a plasma glycoprotein and the only known positive regulator of the complement cascade. It binds and stabilizes the alternative pathway (AP) C3 convertase C3bBb and may also serve as a platform to form new C3bBb convertases on cell surface. Properdin-mediated AP activation plays an important role in host defense, and properdin deficiency (PFD) increases the risk for Neisseria meningitidis infection in human. Properdin-deficiency exacerbated disease condition in a murine model of factor H- (fH-) related C3 glomerulopathy, but prevented the development of allergen-induced airway inflammation. Human properdin is produced with a signal peptide sequence (a.a. 1-27), which is removed posttranslationally to yield the mature protein (a.a. 28-469) composed entirely of seven TSP type-1 domains (domain 0 through 6; a.a. 28-462). TSP type-1 domains 0 and 6 bind to each other and mediate multimerization.

Especificidad

Clone 6E11A4 specifically detects human properdin.

Aplicación

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Properdin in human tonsil tissue sections.

ELISA Analysis: A representative lot detected Properdin in ELISA applications (Blatt, A.Z., et. al. (2016). J Immunol. 196(11):4671-4680).

Inhibition Analysis: A representative lot detected Properdin in Inhibition applications (Blatt, A.Z., et. al. (2016). J Immunol. 196(11):4671-4680).

This mouse monoclonal Anti-Properdin, clone 6E11A4 Antibody, Cat. No. MABF2128 is tested for use in Western Blotting, ELISA, and Immunohistochemistry (Paraffin), for the detection of Properdin. Inhibits function in Properdin.

Calidad

Evaluated by Western Blotting in purified human properdin.

Western Blotting Analysis: 1 µg/mL of this antibody detected Properdin in purified human properdin.

Descripción de destino

~51 kDa observed; 51.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Otras notas

Concentration: Please refer to lot specific datasheet.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable