MABE670
Anti-Acetyl Histone H3 (Lys27) Antibody, clone 5E2.2
clone 5E2.2, from mouse
Sinónimos:
H3K27Ac, Histone H3 (acetyl K27), H3 histone family member T, histone cluster 3, H3t
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
5E2.2, monoclonal
reactividad de especies
human
técnicas
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable
isotipo
IgG2bκ
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
ambient
modificación del objetivo postraduccional
acetylation (Lys27)
Información sobre el gen
human ... H3C1(8350)
Descripción general
Histone H3.1t (UniProt: Q16695; also known as H3K27Ac, Histone H3 (acetyl K27), H3 histone family member T, histone cluster 3, H3t) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histones are dynamic proteins that undergo multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone acetylation and deacetylation are reversible and play a major role in determining chromatin structure and function. Histone acetylation occurs mainly on the amino-terminal tail domains of histones. Acetylation neutralizes positive charges on histone and promotes the less condensed and more accessible chromatin conformation known as euchromatin. Histone acetylation is vital in regulating transcriptional activation and DNA replication, recombination, and repair. Histone H3 is one of the five ain histone proteins involved in the structure of chromatin in eukaryotic cells. It is the most extensively modified of the five histones. It features a main globular domain and a long N-terminal tail. Histone H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
Especificidad
Clone 5E2.2 detects histone H3 acetylated on lysine27 in human.
Inmunógeno
KLH-cojugated peptide corresponding to a sequence around lysine 27 of yeast histone H3.
Aplicación
Acetyl Histone H3 (Lys27) Antibody, clone 5E2.2, Cat. No. MABE670, is a highly specific mouse monoclonal antibody, that targets Acetyl Histone H3 (Lys27) and has been tested in Western Blotting and Immunocytochemistry and Dot Blot.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Acetyl Histone H3 (Lys27) in Hela cells.
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected Acetyl Histone H3 (Lys27) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected Acetyl Histone H3 (Lys27) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Calidad
Evaluated by Western Blotting in HeLa cells stimulated with Sodium Butyrate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Acetyl Histone H3 (Lys27) in 10 µg of HeLa cells stimulated with Sodium Butyrate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Acetyl Histone H3 (Lys27) in 10 µg of HeLa cells stimulated with Sodium Butyrate.
Descripción de destino
~17 kDa observed; 15.5 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Ligadura / enlace
Replaces: 05-1334
Forma física
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Almacenamiento y estabilidad
Stable for 1 year at 2-8°C from date of receipt.
Otras notas
Concentration: Please refer to lot specific datasheet.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Certificados de análisis (COA)
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Xiangxiang Wei et al.
Science advances, 5(3), eaau7887-eaau7887 (2019-03-21)
The transcription factor BTB and CNC homology 1 (Bach1) is expressed in the embryos of mice, but whether Bach1 regulates the self-renewal and early differentiation of human embryonic stem cells (hESCs) is unknown. We report that the deubiquitinase ubiquitin-specific processing
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