MABE1933

Anti-ASXL1 Antibody, clone 12F9

• Código UNSPSC: 12352203

Propiedades

• origen biológico: rat
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 12F9, monoclonal
• mol peso: calculated mol wt 165.14 kDa; observed mol wt ~165 kDa
• purificado por: using protein G
• reactividad de especies: human
• envase: antibody small pack of 100 μg
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG2aκ
• secuencia del epítopo: N-terminal
• Nº de acceso Protein ID: NP_056153
• Nº de acceso UniProt: Q8IXJ9
• Condiciones de envío: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ASXL1(171023)

Descripción

Descripción general

Polycomb group protein ASXL1 (UniProt: Q8IXJ9; also known as Additional sex combs-like protein 1) is encoded by the ASXL1 (also known as KIAA0978) gene (Gene ID: 171023) in human. ASXL1 is a member of the ASXL family that is expressed at low levels in various tissues. However, higher expression is reported in testes. It is a member of the ASXL family with a highly conserved ASX homology (ASXH) domain at the N-terminal region and a plant homeodomain (PHD) finger (aa 1503-1540) at the C-terminal region. The ASXH domain mediates its interaction with a partner protein BAP1, which is an essential component of the polycomb repressive deubiquinase complex (PR-DUB), in which it is involved in deubiquitination of monoubiquitinated histone H2A at lysine 119 (H2AK119ub). ASXL1 also acts as a sensor of N6-methyladenosine methylation on DNA (m6A). It recognizes and binds m6A DNA, leading to its ubiquitination and degradation by TRIP12, thereby inactivating the PR-DUB complex and regulating polycomb silencing. Its HARE-HTH-type domain (aa 11-86) recognizes and binds m6A. Mutations in ASXL1 gene are known to perturb development and cause Bohring-Opitz syndrome that is characterized by severe intrauterine growth retardation, poor feeding, and mental retardation. Some mutations are also observed in myeloid neoplasms, including myelodysplastic syndromes, chronic myelomonocytic leukemia, and acute myeloid leukemia and are associated with poor prognosis. (Ref.: Matheus, F., et al. (2019). Stem Cell Reports 12(5); 861-868; Asada, S., et al. (2018). Nat. Commun. 9: Article 2733; Scheuermann, JC., et al. (2010). Nature. 465(7295); 243-247).

Especificidad

Clone 12F9 is a rat monoclonal antibody that detects human Polycomb group protein ASXL1.

Inmunógeno

His-tagged recombinant fragment corresponding to the first 618 amino acids from the N-terminal region of human Polycomb group protein ASXL1.

Aplicación

Quality Control Testing

Evaluated by Western Blotting in Human spleen tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ASXL1 in Huma spleen tissue lysate.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected ASXL1 in Immunocytochemistry applications (Matheus, F.. et al. (2019). Stem Cell Reports. 12(5):861-868).

Western Blotting Analysis: A representative lot detected ASXL1 in Western Blotting applications (Matheus, F.. et al. (2019). Stem Cell Reports. 12(5):861-868).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-ASXL1, clone 12F9, Cat. No. MABE1933, is a rat monoclonal antibody that detects ASXL1 and is tested for use in Immunocytochemistry and Western Blotting.

Forma física

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable